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Table 2 sfGFP fluorescence of different bacterial species carrying pBACOV-sfGFP relative to B. subtilis RIK1285 pBACOV-sfGFP

From: Transmating: conjugative transfer of a new broad host range expression vector to various Bacillus species using a single protocol

Species Relative fluorescence [%]
pBACOV-sfGFP wild-type
B. mojavensis 383 ± 5.1 n. d.
B. vallismortis 239 ± 5.9 0.9 ± 0.1
B. licheniformis 160 ± 8.8 n. d.
B. subtilis 100 ± 0.6 n. d.
B. pumilus 56.1 ± 0.9 n. d. *
B. pseudomycoides 41.0 ± 1.5 n. d.
P. polymyxa 16.7 ± 0.5 3.4 ± 0.2
B. mycoides 14.9 ± 0.1 3.1 ± 0.0
B. sonorensis n. d. 1.1 ± 0.1
  1. Strains were grown for 24 h at 37 °C, 180 rpm (B. mycoides: 72 h at 30 °C, 180 rpm) in SM-Cas medium with supplements, if appropriate. For details, see Material & Methods. Fluorescence was detected with cultures diluted to OD600 = 1 and the fluorescence intensity (excitation: 470 nm, emission 520 nm) and the OD600 were determined. The relative fluorescence (fluorescence intensity/OD600) was calculated by normalizing the measured fluorescence intensity by the OD600. The relative fluorescence of B. subtilis RIK1285 pBACOV-sfGFP was defined as 100%. The given values are mean values ± standard deviation of triplicate measurements. The asterisk * indicates that the B. pumilus wild-type culture did not reach an OD600 of 1 within the 24 h of incubation and therefore was not diluted for fluorescence measurements. n. d.: not detectable (defined as relative fluorescence < 0.7%)