From: Mutations of DnaA-boxes in the oriR region increase replication frequency of the MiniR1–1 plasmid
Plasmid | Location of mutation | Sequence | |
---|---|---|---|
Before mutagenesis | After mutagenesis | ||
pbox1CA-TG | DnaA-box1 | TTATCCACA | TTATCtgCA (DnaA-box1a) |
pbox1TT-GG | DnaA-box1a | TTATCtgCA | ggATCtgCA |
pbox3TA-GG | DnaA-box3 | TTATACATG | TTAggCATG |
pORF2M-V | DnaA-box4 | TTATGCTGA | TTgTGCTGA |
pbox8TT-GG | DnaA-box8 | TCTTACACA | TCggACACA |
pORF111L-W | ORF1 | TTGATT | TgGATT |