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Fig. 6 | BMC Microbiology

Fig. 6

From: The PhyR homolog RSP_1274 of Rhodobacter sphaeroides is involved in defense of membrane stress and has a moderate effect on RpoE (RSP_1092) activity

Fig. 6

a Levels of rpoE mRNA as determined by real time RT PCR in wild type 2.4.1 (white bars), phyR mutant (black bars) and complemented strain (grey bars). The fold change of 7 min versus 0 min under t-BOOH after normalization to rpoZ mRNA level is shown. b Levels of relative expression are shown for catA in response to hydrogen peroxide and gloA, gloB in response to 1O2 exposure in wild type 2.4.1 (white bars), phyR mutant (black bars) and complemented strain (grey bars). Exposure to hydrogen peroxide or 1O2 was performed for 7 min. Values for relative expression levels represent the increase in gene expression compared to that of the control at time point 0 min and were normalized to mRNA levels determined for rpoZ. The mean of three experiments with standard deviation is shown. c Relative rpoE mRNA levels under different stress conditions as determined by real time RT PCR in the wild type and the mutant lacking PhyR. * indicate a significant change (P ≤ 0.05), ** indicate a highly significant change (P ≤ 0.01) according to Students t-test

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