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Fig. 2 | BMC Microbiology

Fig. 2

From: Identification of novel genes in the carotenogenic and oleaginous yeast Rhodotorula toruloides through genome-wide insertional mutagenesis

Fig. 2

Factors affecting transformation efficiency. a pH of induction medium (IM). IM pH was adjusted with 1.0 M hydrogen chloride. b Agar concentration of IM. c Ammonium sulfate concentration in IM. d Different membrane types. e Representative transformation on selected membranes. Membrane-free: transformation conducted without supporting membrane. R. toruloides ATCC 10657 was used as the T-DNA recipient. TFE is presented as the relative transformation efficiency, where the highest value under each tested condition was set to 100%. Each condition was analyzed in triplicates. Error bars represent standard derivations. The induction medium was pH 5.5. Abbreviations of membranes: N, Nylon Hybond N membrane; CA, Cellulose acetate membrane; N+, Nylon Hybond N+ membrane; MF, membrane-free

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BMC Microbiology

ISSN: 1471-2180

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