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Table 3 Plasmids used in this study

From: The distinctive cell division interactome of Neisseria gonorrhoeae

Plasmid

Relevant genotype

Source/Reference

pcIp22

pC132 derivative carrying N-terminal end of P22 repressor

[30]

pcI434

pACYC177 derivative carrying N-terminal end of 434 repressor

[30]

pcIp22-A

pcIp22 derivative carrying the ftsA Ng gene

This study

pcI434-A

pcI434 derivative carrying the ftsA Ng gene

This study

pcIp22-K

pcIp22 derivative carrying the ftsK Ng gene

This study

pcI434-K

pcI434 derivative carrying the ftsK Ng gene

This study

pcIp22-Q

pcIp22 derivative carrying the ftsQ Ng gene

This study

pcI434-Q

pcI434 derivative carrying the ftsQ Ng gene

This study

pcIp22-I

pcIp22 derivative carrying the ftsI Ng gene

This study

pcI434-I

pcI434 derivative carrying the ftsI Ng gene

This study

pcIp22-W

pcIp22 derivative carrying the ftsW Ng gene

This study

pcI434-W

pcI434 derivative carrying the ftsW Ng gene

This study

pcIp22-N

pcIp22 derivative carrying the ftsN Ng gene

This study

pcI434-N

pcI434 derivative carrying the ftsN Ng gene

This study

pcIp22-Z

pcIp22 derivative carrying the ftsZ Ng gene

[32]

pcI434-Z

pcI434 derivative carrying the ftsZ Ng gene

[32]

pcIp22-AT1

pcIp22 derivative carrying the ftsA Ng gene fragment encoding amino acids 1–162

[33]

pcIp22-AT2

pcIp22 derivative carrying the ftsA Ng gene fragment encoding amino acids 1–194

[33]

pcIp22-AT3

pcIp22 derivative carrying the ftsA Ng gene fragment encoding amino acids 1–230

[33]

pcIp22-AT4

pcIp22 derivative carrying the ftsA Ng gene fragment encoding amino acids 231–301

[33]

pcIp22-AT5

pcIp22 derivative carrying the ftsA Ng gene fragment encoding amino acids 302–414

[33]

pcIp22-AT6

pcIp22 derivative carrying the ftsA Ng gene fragment encoding amino acids 351–414

[33]

pcI434-AT1

pcI434 derivative carrying the ftsA Ng gene fragment encoding amino acids 1–162

[33]

pcI434-AT2

pcI434 derivative carrying the ftsA Ng gene fragment encoding amino acids 1–194

[33]

pcI434-AT3

pcI434 derivative carrying the ftsA Ng gene fragment encoding amino acids 1–230

[33]

pcI434-AT4

pcI434 derivative carrying the ftsA Ng gene fragment encoding amino acids 231–301

[33]

pcI434-AT5

pcI434 derivative carrying the ftsA Ng gene fragment encoding amino acids 302–414

[33]

pcI434-AT6

pcI434 derivative carrying the ftsA Ng gene fragment encoding amino acids 351–414

[33]

pET30a

KanR PT7::6Xhis

EMD Millipore, Billerica, MA

pETA

pET30a derivative carrying the ftsA Ng gene

This study

pETQ

pET30a derivative carrying the ftsQ Ng gene

This study

pETZ

pET30a derivative carrying the ftsZ Ng gene

This study

pGEX2T

AmpRPtac::gst::lacIq

Amersham Bioscience, Uppsala, Sweden

pGEXA

pGEX2T derivative carrying the ftsA Ng gene

This study

pGEXN

pGEX2T derivative carrying the ftsA Ng gene

This study