Table 1 Bacterial strains used in this study

E. coli DH5α

supE44 ΔlacU169 (80lacZΔM15) hsdR17 endA1 gyrA96 thi-1 relA1

Gibco

E. coli XL1-Blue

recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB laclq ZΔM15] Tn10

Stratagene

E. coli BL21(DE3)

F⁻ , dcm Δ, ompT, hsdS (r⁻ _B m⁻ _B), gal, λ(DE3)

Stratagene

E. coli C41 (DE3)

F⁻ ompT hsdSB (r⁻ _B m⁻ _B) gal dcm (srl-recA) 306::Tn10 (Tet^r) (DE3)

[70]

E. coli R721

supE thy D(lac-proAB) F′ [proAB ⁺ lacI ^q lacZDM15] glpT::O-_P434/P22 lacZ

[30]

N. gonorrhoeae CH811

Auxotype (A)/serotype (S)/plasmid content (P) class: nonrequiring/IB-2/plasmid-free, Str^r

[71]