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Table 1 Organisms and plasmids used in this study

From: Characterization of lpaH2 gene corresponding to lipopeptide synthesis in Bacillus amyloliquefaciens HAB-2

Organisms and plasmids Description Source
Escherichia coli DH5α SupE44 ∆ lacU 169 Ø 80 lacZ ∆ M15 hsdR17 recA1 gyrA96 thi-1 relAF Transgen Bio Inc.
Bacillus amyloliquefaciens HAB-2 lpaH2 gene, wild type, produces lipopeptides Collection of this lab
Bacillus amyloliquefaciens HABlpa deficient in lipopeptides; Cmr, Ampr This work
Bacillus subtilis 168 deficient in lipopeptides Collection of this lab
Bacillus subtilis 168 lpa lpaH2 gene, can produce lipopeptides; Cmr, Ampr This work
Colletotrichum gloeosporioides Indicator strain, causative agent of mango anthracnose Collection of this lab
pAD 43–25 plasmid Shuttle vector carrying GFP; Cmr Collection of this lab
pMD18-T plasmid T-clone site vector; lacZ; Ampr TaKaRa Bio Inc.
  1. Amp r ampicillin resistance, Cm r chloramphenicol resistance, GFP green fluorescent protein