Table 1 Organisms and plasmids used in this study
Organisms and plasmids | Description | Source |
|---|---|---|
Escherichia coli DH5α | SupE44 ∆ lacU 169 Ø 80 lacZ ∆ M15 hsdR17 recA1 gyrA96 thi-1 relAF− | Transgen Bio Inc. |
Bacillus amyloliquefaciens HAB-2 | lpaH2 gene, wild type, produces lipopeptides | Collection of this lab |
Bacillus amyloliquefaciens HABâ–³lpa | deficient in lipopeptides; Cmr, Ampr | This work |
Bacillus subtilis 168 | deficient in lipopeptides | Collection of this lab |
Bacillus subtilis 168 lpa | lpaH2 gene, can produce lipopeptides; Cmr, Ampr | This work |
Colletotrichum gloeosporioides | Indicator strain, causative agent of mango anthracnose | Collection of this lab |
pAD 43–25 plasmid | Shuttle vector carrying GFP; Cmr | Collection of this lab |
pMD18-T plasmid | T-clone site vector; lacZ; Ampr | TaKaRa Bio Inc. |