msaABCR operon is involved in persister cell formation in Staphylococcus aureus

BMC Microbiology

Table 4 Comparison of the rate of persister cell formation between the msaABCR deletion mutant and wildtype USA300 LAC

Fold change in persister cells formation (mutant vs. wildtype)
Antibiotics	Exponential Growth phase	Stationary Growth phase	Biofilm
DAP	UD**	- 2.15	- 747.11**
VAN	- 23.82**	- 1.40	- 2.8X10⁴**
RIF	- 54.16**	- 1.66	- 19.56
LIN	- 1.18	- 44.67**	- 14.24**
GEN	- 12.50**	UD**	- 2.33
DAP + RIF	- 25.03**	- 23.02**	UD**
VAN + RIF	- 96.12**	- 4.04	- 32.35**
LIN + RIF	1.47	- 3.35	- 4.32
DAP + GEN	- 55.30*	- 53.88**	- 0.73
VAN + GEN	- 13.23**	- 0.88	- 406.66**
LIN + GEN	+ 14.43**	- 9.43**	- 37.50**

DAP: daptomycin, VAN: vancomycin, RIF-rifampicin, LIN: linezolid, GEN: gentamicin, UD: undetectable persister cells in the msaABCR deletion mutant
Statistically significant (* p-value <0.05, ** p-value <0.005): The difference in CFUs observed in the mutant cells Vs wild type cells was analyzed by students T-test to measure the statistical significance. All the CFUs experiments that involved the biofilm cells were analyzed by one-way ANOVA followed by post-hoc Tukey’s HSD test to measure the statistical significance

ISSN: 1471-2180