Fig. 3

Expression of bb0170 to bb0176 under decreased-oxygen conditions. Gene expression of the Bat-like genes is quantified under atmospheric oxygen and decreased oxygen conditions. The expression of each gene is determined by comparing the expression under atmospheric oxygen to the expression under low-oxygen growth conditions after normalization to the endogenous control gene, flaB (ΔCT = Ct (goi) – Ct (ref); where “goi” refers to the gene of interest, and “ref” to the reference gene used in the study). All genes tested were found to increase expression under low-oxygen conditions except for bb0174 and rpoN, which both became undetectable in the low-oxygen condition, as denoted by the diamond (♦). For this experiment, rpoN, rrp1, hpk1, and rrp2 have been included as control genes. Statistical analysis was performed using the Holm-Sidak multiple comparisons test with a 95% confidence interval. Each gene was evaluated in triplicate. *Denotes statistical differences: *P value <0.05, **P value <0.01, ***P value <0.001 and ****P value <0.0001