Fig. 4
From: Phosphate signaling through alternate conformations of the PstSCAB phosphate transporter
Induction of alkaline phosphatase expression upon Pi starvation. The Pi concentrations of the growth medium are represented by dashed lines, cell growth as measured by OD600 readings are shown with solid lines, and Pi-signaling, as measured by AP expression, is shown with solid vertical bars. The experiments reported in this figure were repeated three times on different days with similar results and we present the data from one trial. Measurements represent the averages of two technical replicates ± SD. a Wild type strain, BW25113 (b) BW26337 strain (ΔpstSCABphoU) carrying either pRR48 plasmid (Red) or p48SCABU (Blue). (c) BW26337 strain carrying either p48SCAB(Q160K)U (Blue) or p48SCAB(E179Q)U (Red)