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Fig. 6 | BMC Microbiology

Fig. 6

From: Transcript profiling of the immunological interactions between Actinobacillus pleuropneumoniae serotype 7 and the host by dual RNA-seq

Fig. 6

Comparison of relative expression levels measured by RNA-seq and qRT-PCR. qRT-PCR (mean): the mean of log2 (fold change) of six RNA samples. qRT-PCR0: the RNA sample used for sequencing. qRT-PCR 1–5: RNA samples mixed in equal parts for sequencing. Of the genes that were found to exhibit significant difference in expression by RNA-seq, the following were selected for detection by qRT-PCR: adhE, dmsA, dmsB, fruk, malE, lamb, hiuH, metQ1 of App, and cxcl2, csf3, ccl4, ccl3, Tpm3, 60SrDNA, Csnk2a1 and Hbα of Mmu. A strong correlation (R2 = 0.989) in gene expression was found between the RNA-seq and qRT-PCR results

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