Fig. 6
![Fig. 6](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12866-017-1105-4/MediaObjects/12866_2017_1105_Fig6_HTML.gif)
Comparison of relative expression levels measured by RNA-seq and qRT-PCR. qRT-PCR (mean): the mean of log2 (fold change) of six RNA samples. qRT-PCR0: the RNA sample used for sequencing. qRT-PCR 1–5: RNA samples mixed in equal parts for sequencing. Of the genes that were found to exhibit significant difference in expression by RNA-seq, the following were selected for detection by qRT-PCR: adhE, dmsA, dmsB, fruk, malE, lamb, hiuH, metQ1 of App, and cxcl2, csf3, ccl4, ccl3, Tpm3, 60SrDNA, Csnk2a1 and Hbα of Mmu. A strong correlation (R2 = 0.989) in gene expression was found between the RNA-seq and qRT-PCR results