| Roche 454 | Ion Torrent | Illumina MiSeq |
---|---|---|---|
Sequencing Kit | GS FLX Titanium XLR70 | PGM 400 Sequencing | MiSeq Reagent Kits v2 |
Expected Read Length | Up to 600 bp | Up to 400 bp | MiSeq Reagent Kit v2: Up to 2 × 250 bp |
Typical Throughput | 450Â Mb | Ion 314â„¢ Chip v2: Up to 100Â Mb Ion 316â„¢ Chip v2: Up to 1 Gb Ion 318â„¢ Chip v2: Up to 2 Gb | Up to 8.5 Gb |
Reads per Run | ~1000,000 shotgun, ~700,000 amplicon | Ion 314™ Chip v2: 400–550 thousand Ion 316™ Chip v2: 2–3 millions Ion 318™ Chip v2: 4–5.5 millions | ~15 million reads |
Consensus Accuracy | 99.995% | 99% | 99% |
Run Time | 10Â h | Ion 314â„¢ Chip v2: 2.3 to 3.7Â h Ion 316â„¢ Chip v2: 3.0 to 4.9Â h Ion 318â„¢ Chip v2: 4.4 to 7.3Â h | 4Â h and approximately 39Â h depending on the number of cycles |
Sample Input | gDNA, cDNA, or amplicons (PCR products) | gDNA, cDNA, or amplicons (PCR products) | gDNA, cDNA, or amplicons (PCR products) Small genome, amplicon, and targeted gene panel sequencing |
Weight | 532 lbs. (242Â kg) | 65 lbs. (30Â kg) | 120 lbs. (54.5Â kg) |
Instrument cost | ~$500Â K | ~ $80Â k | ~ $125Â k |