Table 2 E. coli strains and plasmids used in this study
Strain / plasmid | Relevant genotype | Reference or source |
|---|---|---|
MG1655 | F-lambda- ilvG- rfb-50 rph-1 | Blattner et al. (1997) [68] |
EMC07E | F-lambda- ilvG- rfb-50 rph-1 cpxAR::kan | Surmann et al. (2016) [34] |
GP01E | F-lambda- ilvG- rfb-50 rph-1, arcA-Snap | this work |
BTH-101 | F-, cya-99, araD139, galE15, galK16, rpsL1, (Str r), hsdR2, mcrA1, mcrB1 | Euromedex, Souffelweyersheim, France |
pTnlpE | nlpE cloned in pTrc99A, AmpR | Zhou et al. (2011) [24] |
pKT01E | CpxA-Strep on pMal-p2X without MalE, AmpR | Tschauner et al. (2014) [49] |
pUT18 | T18-fragment (amino acids 225–399 of cyaA), lac Promotor, MCS located at 5′-end of T18; in pUC19 (high copy number), AmpR | Euromedex |
pKT25 | T25-fragment (amino acids 1–224 of cyaA), lac Promotor, MCS located at 3′-end of T25; in pSU40 (low copy number), KanR | Euromedex |
pSH106E | cpxA in pUT18, AmpR | this work |
pSH105E | arcB in pUT18, AmpR | this work |
pEL16E | cpxR in pKT25, KanR | this work |
pGP02E | arcA in pKT25, KanR | this work |
pKT25-zip | leucine zipper of GCN4 from Saccharomyces cerevisiae in pKT25, KanR | Euromedex |
pUT18C-zip | leucine zipper of GCN4 from Saccharomyces cerevisiae in pUT18C, AmpR | Euromedex |