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Table 2 E. coli strains and plasmids used in this study

From: The role of the two-component systems Cpx and Arc in protein alterations upon gentamicin treatment in Escherichia coli

Strain / plasmid Relevant genotype Reference or source
MG1655 F-lambda- ilvG- rfb-50 rph-1 Blattner et al. (1997) [68]
EMC07E F-lambda- ilvG- rfb-50 rph-1 cpxAR::kan Surmann et al. (2016) [34]
GP01E F-lambda- ilvG- rfb-50 rph-1, arcA-Snap this work
BTH-101 F-, cya-99, araD139, galE15, galK16, rpsL1,
(Str r), hsdR2, mcrA1, mcrB1
Euromedex, Souffelweyersheim, France
pTnlpE nlpE cloned in pTrc99A, AmpR Zhou et al. (2011) [24]
pKT01E CpxA-Strep on pMal-p2X without MalE, AmpR Tschauner et al. (2014) [49]
pUT18 T18-fragment (amino acids 225–399 of cyaA), lac Promotor, MCS located at 5′-end of T18; in pUC19 (high copy number), AmpR Euromedex
pKT25 T25-fragment (amino acids 1–224 of cyaA), lac Promotor, MCS located at 3′-end of T25; in pSU40 (low copy number), KanR Euromedex
pSH106E cpxA in pUT18, AmpR this work
pSH105E arcB in pUT18, AmpR this work
pEL16E cpxR in pKT25, KanR this work
pGP02E arcA in pKT25, KanR this work
pKT25-zip leucine zipper of GCN4 from Saccharomyces cerevisiae in pKT25, KanR Euromedex
pUT18C-zip leucine zipper of GCN4 from Saccharomyces cerevisiae in pUT18C, AmpR Euromedex