Fig. 1

Cryptoccocus yeasts stained with Trypan blue. a Image of heat killed Cryptococcus yeasts stained with trypan blue and visualized by light microscopy. The dead cells appear dark/black. b Image of Cryptococcus yeasts left at room temperature and stained with trypan blue. The live cells appear light/white. c Image of a patient’s CSF sample stained with trypan blue. There is a mixed population of dead and live cells. d Cryptococcus isolate grown on media containing SDA, chloramphenicol and trypan blue. The colonies appear dark blue. “Scale bar = 10 μm”