Fig. 1

Effects of PepO on IL-8 and IP-10 synthesis and release by activated BEAS-2B. a After treated with medium, GST or PepO (10 μg /ml) for 24 h, IL-8 and IP-10 production in cell culture medium of BEAS-2B were determined with ELISA. b Quantitative RT-PCR were used to measure IL-8 and IP-10 mRNA in cells activated by PepO (10 μg /ml) for indicated times; (c,d) ELISA was used to detected IL-8 and IP-10 release in culture medium of cells stimulated by different concentration of PepO for indicated times. Results are expressed as the mean ± SD (n = 3). Student’s t-test was performed to calculate the statistical significance (*p < 0.05 or **p < 0.01***p < 0.001)