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Fig. 2 | BMC Microbiology

Fig. 2

From: Environmentally triggered genomic plasticity and capsular polysaccharide formation are involved in increased ethanol and acetic acid tolerance in Kozakia baliensis NBRC 16680

Fig. 2

Morphology and genetic switch of K. baliensis NBRC 16680 wild-type during cultivation in different media (a) In (a), growth of K. baliensis NBRC 16680 on NaG agar plates, plated at time point one after inoculation in NaG medium (0 h). Growth of K. baliensis NBRC 16680 on NaG agar plates, plated after 48 h of incubation in NaG medium (b), NaG-AA medium (c) and NaG-EtOH medium (d). Rough mutant colonies (ΔgumD) are indicated via a white arrow. Wild-type colonies are marked with a grey arrow. b) Shows a section of the gum-cluster of K. baliensis NBRC 16680 with the genomic location of the gumD gene (1471468–1,472,730 bp), oxidoreductase gene (ox, 1,469,488–1,470,627 bp) and a gene coding for a hypothetical protein (hp, 1,467,916–1,469,289 bp), based on JU Brandt, Jakob, F., Behr, J., Geissler, A.J., Vogel, R.F. [20]. Random colony PCRs of the respective rough colonies were carried out, targeting the transposon insertion side, using a genomic primer (G4F_Fw) and a primer, targeting the mobile element (TE_Rv). PCR products were subsequently sequenced. (c) Shows a schematic representation of the transposon insertion at the gumD locus in the rough colonys of K. baliensis NBRC 16680. The mobile element (me) is shown as grey bar with the corresponding insertion locus written in bold blue. The frequency of the found insertion site is marked on the left side, as n = x

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BMC Microbiology

ISSN: 1471-2180

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