Table 4 List of randomly chosen bacterial IMPase-like proteins that are most likely wrongly annotated with a focus on HolPases. All proteins have been compared to the five IMPase-like proteins in C. glutamicum and reannotated according to the detected motifs
Organism | GI-number | current annotation | protein with highest similarity in C. glutamicum | presence of expected motifs | similarity to HisN Cg (BlastP score) [bits] | new functional assignment |
|---|---|---|---|---|---|---|
Class 1 | ||||||
  Pseudomonas pseudoalcaligenes | 489546409 | IMPase | HisN Cg | yes | 117 | HisN |
  Rudaea cellulosilytica | 648601632 | IMPase | HisN Cg | yes | 108 | HisN |
  Marinobacter psychrophilus | 860341079 | IMPase | HisN Cg | yes | 110 | HisN |
  Rubrobacter radiotolerans | 627778235 | FBPase- or IMPase | HisN Cg | yes | 123 | HisN |
Class 2 | ||||||
  Streptosporangium roseum | 502655523 | IMPase | HisN Cg | no | 120 | - |
  Rubrobacter xylanophilus | 499884516 | IMPase | HisN Cg | no | 120 | - |
Class 3 | ||||||
  Sediminibacterium salmoneum | 739492005 | HolPase | SuhB Cg | yes | 102 | SuhB |
  Flavihumibacter solisilvae | 743029970 | HolPase | SuhB Cg | yes | 91 | SuhB |
  Desulfatibacillum aliphaticivorans | 654864062 | HolPase | SuhB Cg | yes | 102 | SuhB |
  Leptospirillum ferriphilum | 738123182 | HolPase | SuhB Cg | yes | 89 | SuhB |