Fig. 3

Nitroreductase activity of AzoA, EF0404, EF0648, EF0655, EF1181: native (a) and without prosthetic group enzymes (b). Nitroreductase activity is evaluated by following fluorescence intensity at 365/440 nm during 900 min in presence of 100 μmol.l⁻¹ 7NCCA, 500 μmol.l⁻¹ NADPH and 10 mg.l⁻¹ of indicated enzyme. a The proteins used were purified with their prosthetic group in their native form. No FMN was added for the reaction. b The proteins used were purified without their prosthetic group, that is purified using the denaturing/renaturing protocol. The reduction is followed without addition of FMN (−) and with 5 μmol.l⁻¹ of FMN (− •)