| K99 | F41 | F18 | F6 | K88 |
---|
Tm(°C) | 74.7 ± 0.06 | 76.5 ± 0.12 | 77.6 ± 0.35 | 79.5 ± 0.78 | 80.5 ± 0.15 |
E a
| 1.87 | 1.74 | 1.7 | 1.85 | 1.84 |
r1
2 b
| 0.99 | 0.99 | 0.99 | 0.99 | 0.99 |
DNA range | 1–20% | 0.2%–16.7% | 1%–100% | 0.3%–4.8% | 4.8%–60% | 4.8%–20% |
Slope c
| 2.96 | 2.74 | 2.39 | 0.48 | 1.44 | 1.49 |
r2
2 d
| 0.97 | 0.97 | 0.98 | 0.97 | 0.99 | 0.97 |
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aprimer efficiency determined by individual PCR
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br1
2, correlation coefficient for standard curve determined by individual qPCR
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cSlope for calibration curve correlating the area of the melting peaks area to the proportion of the template DNA
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dr2
2, correlation coefficient for standard curve correlating the area of the melting peaks to the proportion of the template DNA