Table 2 Primers used for qPCR and multiplex HRM-qPCR analysis
Target gene | Sequence (5′--3′) (name) | Size (bp) | TA(°C)a | Reference |
|---|---|---|---|---|
K99 fimbriae (fan A) | CACTTGAGGGTATATGCGATCTT (K99 F) | 92 | 62 | This study |
GACCTCAGTCACAGCAACTATAC (K99 R) | ||||
F41 fimbriae Sub-unit A | GACCTCAGTCACAGCAACTATAC (F41 F) | 110 | 62 | This study |
CGACCCGCAACATCCTTATT (F41 R) | ||||
F18 fimbriae (Fed A) | GGAGGTTAAGGCGTCGAATAG (F18 F) | 90 | 62 | This study |
CCACCTTTCAGTTGAGCAGTA (F18 R) | ||||
F6 fimbriae (Fas A) | GTTCCAGCCTCCAATGATACT (F6 F) | 128 | 62 | This study |
GAAAGAGCTAATCCGCCATTTG (F6 R) | ||||
K88 fimbriae (fae G) | GCACATGCCTGGATGACTGGTG (K88 F) | 439 | 63 | |
CGTCCGCAGAAGTAACCCCACCT (K88 R) | ||||
E. coli (Universal stress protein A) | CCGATACGCTGCCAATCAGT (UspA F) | 884 | 66 | |
ACGCAGACCGTAGGCCAGAT (UspA R) | ||||
Heat-labile toxin | CCGTGCTGACTCTAGACCCCCA (LT F) | 480 | 68 | |
CCTGCTAATCTGTAACCATCCTCTGC (LT R) | ||||
Heat-stable toxins a | ATGAAAAAGCTAATGTTGGC (STa F) | 193 | 65 | |
TACAACAAAGTTCACAGCAG (STa R) | ||||
Heat-stable toxins b | TGCCTATGCATCTACACAAT (STb F) | 113 | 60 | |
CTCCAGCAGTACCATCTCTA (STb R) |