Skip to main content

Table 5 Multiplex assay accuracy for CHIKV and DENV

From: Simultaneous detection of chikungunya virus, dengue virus and human pathogenic Leptospira genomes using a multiplex TaqMan® assay

EQA ID Our laboratory multiplex assay
Ct
Reference laboratory assay
Ct
All enrolled laboratory in SEGA network
Ct
Bias against reference laboratory Bias against all enrolled laboratories in SEGA network
CHIKV
 EQA-SEGA-05-01 23.98 23.19 26.53 +3.41% -9.61%
 EQA-SEGA-05-05 25.04 26.50 26.70 - 5.51% -6.22%
 EQA-SEGA-05-08 22.94 22.06 24.93 +3.99% −7.98%
 Mean 23.99 23.92 26.05 +0.29% -7.91%
DENV
 EQA-SEGA-05-03 27.51 30.13 28.28 −8.70% -2.72%
 EQA-SEGA-05-04 33.97 36.57 33.23 −7.11% +2.23%
 EQA-SEGA-05-06 26.56 28.56 26.68 −7.00% -0.45%
 EQA-SEGA-05-09 30.26 33.39 30.58 −9.37% −1.05%
 Mean 29.57 32.16 29.69 −8.05% −0.40%
  1. A negative bias against reference laboratory accounts for better performance of our multiplex assay