Table 2 Oligonucleotides used in this study
Oligonucleotide | Sequence (5’ to 3’)a |
|---|---|
0498LA-F (SalI) | CCCGTCGACGAAAGTGCGTCCAGCGTAT |
0498LA-R (BamHI) | CCCGGATCCTTTGCTCTGCACGTTCTGC |
0498RA-F (KpnI) | CCCGGTACCACTTCGCTCAAAATAATCCTAAT |
0498RA-F (NheI) | CCCGCTAGCACCTAAGTCTAAACCTCCACAAC |
Kan-F (BamHI) | CCCGGATCCGAACCATTTGAGGTGATAGG |
Kan-R (KpnI) | CCCGGTACCGGTACTAAAACAATTCATCCAG |
0498-F | GGAAGAACATCTGCTGGAA |
0498-R | AGCTACGATAATTTAGTGCC |
0497-F | TTTGTGGCTGAGAATGTTAA |
0497-R | GTATTGGTGCTTGTCTACCTG |
SpcR-F (KpnI) | CCCGGTACCAGAGTTGGTAGCTCTTGATCCG |
SpecR-R (EcoRI) | CCCGAATTCTTTAAAAGTAAGCACCTGTTATTC |
amyLA-F (SalI) | CCCGTCGACGAATAATGGGCAAATTCAGTGG |
amyLA-R (BamHI) | CCCGGATCCAGAAGCGTCTTGTTCTTGACGA |
amyRA-F (EcoRI) | CCCGAATTCATTTCACAAAGAAAATAGCA |
amyRA-R (NheI) | CCCGCTAGCGGATGTGATTTACCAAAGTCT |
C0498-F (BamHI) | CCCGGATCCGTATTCCAGACTGTTTGGAGCT |
C0498-R (KpnI) | CCCGGTACCCTCTATCTAAATCAGGAGCAGGTT |