Transposon insertion sequencing reveals T4SS as the major genetic trait for conjugation transfer of multi-drug resistance pEIB202 from Edwardsiella

Table 4 Genes identified by TIS in pEIB202 that affects maintenance or conjugation of pEIB202

Loci	Gene	Product	Output/input ^a	P-value
ETAE_p001	virD4	T4SS component VirD4	0.015	0.000548801
ETAE_p002	virB11	T4SS component VirB11	0.0058	0.000531746
ETAE_p003	virB10	T4SS component VirB10	0.00099	0.000531259
ETAE_p004		hypothetical protein	0.0022	0.000517762
ETAE_p005	virB9	T4SS component VirB9	0.0039	0.000520783
ETAE_p006	virB8	T4SS component VirB8	0.0014	0.000530141
ETAE_p007		putative lipoprotein	0.0034	0.000493853
ETAE_p008		hypothetical protein	0.10	0.0003746
ETAE_p009	virB6	T4SS component VirB6	0.011	0.000544597
ETAE_p010		putative lipoprotein	0.18	1.61153E-05
ETAE_p011	virB5	T4SS component VirB5	0.0027	0.000535705
ETAE_p012		hypothetical protein	0.026	0.0003982
ETAE_p013	virB4	T4SS component VirB4	0.0086	0.000541165
ETAE_p014	virB2	T4SS component VirB2	0.019	0.000514194
ETAE_p015		hypothetical protein	0.073	9.96994E-06
ETAE_p016	topA	DNA topoisomerase	4.30	0.000340276
ETAE_p026		transcriptional repressor protein	0.020	6.77947E-06
ETAE_p047	mobC	putative mobilisation protein	0.13	0.000639706
ETAE_p048	virD2	VirD2 component	0.028	0.000545018
ETAE_p053	traC	DNA primase TraC4	0.15	2.38855E-05

^aThe data indicate that the read number of a specific gene in output is significantly under-represented or over-represented as compared to that in input with a cutoff of less than 0.25 or higher than 4-fold and p < 0.001

ISSN: 1471-2180