Skip to main content

Table 2 Limit of detection of HRM assay

From: Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study

Parameter Genus Species a Cluster b gDNA amount (pg)         
1000 500 50 10 5 1 0.5 0.1 0.05 0.01
Detection c Aspergillus creber 3 12/12 12/12 12/12 12/12 12/12 12/12 11/12 6/12 0/12 0/12
  Aspergillus sydowii 2 12/12 12/12 12/12 12/12 12/12 12/12 11/12 9/12 0/12 0/12
  Aspergillus versicolor 1 12/12 12/12 12/12 12/12 12/12 12/12 12/12 12/12 10/12 0/12
Confidence (%) d Aspergillus creber 3 97.98 ± 1.28 96.66 ± 1.54 99.03 ± 0.59 99.30 ± 0.29 99.70 ± 0.02 97.99 ± 0.53 96.65 ± 0.36 97.99 ± 0.53. / /
  Aspergillus sydowii 2 98.72 ± 0.39 99.25 ± 0.26 99.28 ± 1.66 99.67 ± 1.12 98.840 ± 2.34 96.83 ± 3.11 95.63 ± 1.63 95.83 ± 3.11 / /
  Aspergillus versicolor 1 98.99 ± 0.26 99.23 ± 0.35 99.49 ± 2.01 98.93 ± 0.60 99.76 ± 096 98.52 ± 2.32 97.49 ± 0.11 97.60 ± 1.06 96.37 ± 1.540 /
  1. agDNA extracted from pure strains from the BCCM/IHEM collection. BCCM/IHEM strains used as reference: A. creber IHEM 2646, A. sydowii IHEM 26347, A. versicolor IHEM 18884
  2. bCluster defined with the Biorad Precision Melt Analysis software 1.2 (Temse, Belgium)
  3. cDetection: Number of positive signals i.e., correct cluster and confidence >95%, observed for each dilution, the LOD for each species is indicated in bold
  4. dConfidence (%) is the average and standard deviation (±SD)