Table 1 Summary of the purification steps of the cholesterol oxidase produced by Streptomyces aegyptia NEAE 102
Purification step | Total protein content (mg) | Cholesterol oxidase activity | |||
|---|---|---|---|---|---|
Total activity (U) | Specific activity (U/mg protein) | Recovery (%) | Purification fold | ||
Culture filtrate | 3811.27 | 19527.53 | 5.12 | 100 | 1 |
(NH4) 2SO4, post dialysis | 60.57 | 1217.88 | 20.11 | 6.23 | 3.92 |
Ion exchange on DEAE Sepharose CL-6B | 31.15 | 501.05 | 16.08 | 2.56 | 3.14 |