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Table 1 Summary of the purification steps of the cholesterol oxidase produced by Streptomyces aegyptia NEAE 102

From: Purification, characterization and amino acid content of cholesterol oxidase produced by Streptomyces aegyptia NEAE 102

Purification step Total protein content (mg) Cholesterol oxidase activity
Total activity (U) Specific activity (U/mg protein) Recovery (%) Purification fold
Culture filtrate 3811.27 19527.53 5.12 100 1
(NH4) 2SO4, post dialysis 60.57 1217.88 20.11 6.23 3.92
Ion exchange on DEAE Sepharose CL-6B 31.15 501.05 16.08 2.56 3.14