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Fig. 1 | BMC Microbiology

Fig. 1

From: Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems

Fig. 1

Sequencing error rate distribution. a) Error rate distribution along the full-length fragment (421 bp) after analysis of 15,000 reads of L. pneumophila amplified with HotStarTaq (open blue circles) and KAPA HiFi DNA polymerases (red open circles). LOESS curves illustrate the reduced error rate with KAPA HiFi polymerase and the higher error rate in fragment covered by reverse reads. b) Comparison of error rate per base position with HotStarTaq and KAPA HiFi enzymes. A linear regression model explains the relationship between the two variables. Coefficient of determination (R2) of 0.73

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BMC Microbiology

ISSN: 1471-2180

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