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Table 2 Primers used in gene deletion experiments
| Primers | Sequence (5’ to 3’)a |
|---|---|
| For mutagenesis of copR | |
| LPW23977 (copR-UF) | GCTCTAGACCGAAAGGGTTCATTACG |
| LPW23978 (copR-UR) | TCAGCTTGACCTCGAGCCCCTTCTTCAG |
| LPW23979 (copR-DF) | GGGGCTCGAGGTCAAGCTGATCCATACC |
| LPW23980 (copR-DR) | ACATGCATGCTAGCCGTCGAGCAGATC |
| LPW24342 (copR-IF) | GGTTTCAGCGTCGATCTC |
| LPW24485 (copR-IR2) | CATGTCCCATACGTACGA |
| For mutagenesis of lipA | |
| LPW23981 (lipA-UF) | GCTCTAGAACATGCTCGAACGCTGTG |
| LPW23982 (lipA-UR) | CAACGACTGCAATCAGCGCGTTCGATGG |
| LPW23983 (lipA-DF) | CGCGCTGATTGCAGTCGTTGGTCAGTTG |
| LPW23984 (lipA-DR) | CGAGCTCAGCACCTGCATGAACAC |
| LPW24340 (lipA-IF) | GGCATACCCGTCTATGTG |
| LPW24341 (lipA-IR) | GACGATGTTTGCCAACTG |
| For complementation of ∆copR and ∆lipA | |
| LPW29008 (copR-CF) | GGAATTCCATATGCGGCCATGCGCATCCTGATA |
| LPW29009 (copR-CR) | TGCTCTAGACGTCATGCGTCGTCCTTCGG |
| LPW29010 (lipA-CF) | GGGAATTCCATATGCATGAACGTATCGACACGCC |
| LPW29011 (lipA-CR) | CTAGTCTAGATGTGCGGCTACGCCTGATCG |
