Table 2 Primers used in gene deletion experiments
Primers | Sequence (5’ to 3’)a |
|---|---|
For mutagenesis of copR | |
LPW23977 (copR-UF) | GCTCTAGACCGAAAGGGTTCATTACG |
LPW23978 (copR-UR) | TCAGCTTGACCTCGAGCCCCTTCTTCAG |
LPW23979 (copR-DF) | GGGGCTCGAGGTCAAGCTGATCCATACC |
LPW23980 (copR-DR) | ACATGCATGCTAGCCGTCGAGCAGATC |
LPW24342 (copR-IF) | GGTTTCAGCGTCGATCTC |
LPW24485 (copR-IR2) | CATGTCCCATACGTACGA |
For mutagenesis of lipA | |
LPW23981 (lipA-UF) | GCTCTAGAACATGCTCGAACGCTGTG |
LPW23982 (lipA-UR) | CAACGACTGCAATCAGCGCGTTCGATGG |
LPW23983 (lipA-DF) | CGCGCTGATTGCAGTCGTTGGTCAGTTG |
LPW23984 (lipA-DR) | CGAGCTCAGCACCTGCATGAACAC |
LPW24340 (lipA-IF) | GGCATACCCGTCTATGTG |
LPW24341 (lipA-IR) | GACGATGTTTGCCAACTG |
For complementation of ∆copR and ∆lipA | |
LPW29008 (copR-CF) | GGAATTCCATATGCGGCCATGCGCATCCTGATA |
LPW29009 (copR-CR) | TGCTCTAGACGTCATGCGTCGTCCTTCGG |
LPW29010 (lipA-CF) | GGGAATTCCATATGCATGAACGTATCGACACGCC |
LPW29011 (lipA-CR) | CTAGTCTAGATGTGCGGCTACGCCTGATCG |