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Table 1 Bacterial strains and plasmids used in gene deletion experiments

From: Identification and analysis of genomic islands in Burkholderia cenocepacia AU 1054 with emphasis on pathogenicity islands

Strains or plasmids Relative characteristics Source or reference
E. coli DH5α F, Ф80d lacZ∆M15, ∆(lacZYA-argF)U169, endA1, recA1, hsdR17(rk, mk+) deoR, thi-1, supE44, λ, gyrA96(Nalr), relA1 Invitrogen
E. coli SM10(λ pir) thi thr leu tonA lacY supE recA::RP4-2-TC::Mu Km r λpir [53]
B. cenocepacia AU1054 Clinical isolate BCCM-LMBP
AU1054∆copR AU1054 derivative with copR deletion This study
AU1054∆lipA AU1054 derivative with lipA deletion This study
PCRII-TOPO Cloning vector; ori lacZ Km+ Invitrogen
pRK2013 ori colE1, RK2 derivative, KanR, mob +, tra + ATCC
pGPI-SceI ori R6K,TpR, mob +, carries I-SceI cut site [37]
pGPI∆copR pGPI-SceI carrying 5’- and 3’-flanking regions of copR for mutagenesis of copR This study
pGPI∆lipA pGPI-SceI carrying 5’- and 3’-flanking regions of lipA for mutagenesis of lipA This study
pDAI-SceI ori pBBR1,TetR, mob +, P dhfr , FLAG epitope, carries I-SceI cut site [37]
pDA-copR pDAI-SceI with ORF of copR replacement of SceI gene This study
pDA-lipA pDAI-SceI with ORF of lipA replacement of SceI gene This study