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Table 1 Bacterial strains and plasmids used in gene deletion experiments

From: Identification and analysis of genomic islands in Burkholderia cenocepacia AU 1054 with emphasis on pathogenicity islands

Strains or plasmids Relative characteristics Source or reference
Strains
E. coli DH5α F, Ф80d lacZ∆M15, ∆(lacZYA-argF)U169, endA1, recA1, hsdR17(rk, mk+) deoR, thi-1, supE44, λ, gyrA96(Nalr), relA1 Invitrogen
E. coli SM10(λ pir) thi thr leu tonA lacY supE recA::RP4-2-TC::Mu Km r λpir [53]
B. cenocepacia AU1054 Clinical isolate BCCM-LMBP
AU1054∆copR AU1054 derivative with copR deletion This study
AU1054∆lipA AU1054 derivative with lipA deletion This study
Plasmids
PCRII-TOPO Cloning vector; ori lacZ Km+ Invitrogen
pRK2013 ori colE1, RK2 derivative, KanR, mob +, tra + ATCC
pGPI-SceI ori R6K,TpR, mob +, carries I-SceI cut site [37]
pGPI∆copR pGPI-SceI carrying 5’- and 3’-flanking regions of copR for mutagenesis of copR This study
pGPI∆lipA pGPI-SceI carrying 5’- and 3’-flanking regions of lipA for mutagenesis of lipA This study
pDAI-SceI ori pBBR1,TetR, mob +, P dhfr , FLAG epitope, carries I-SceI cut site [37]
pDA-copR pDAI-SceI with ORF of copR replacement of SceI gene This study
pDA-lipA pDAI-SceI with ORF of lipA replacement of SceI gene This study