Table 1 Bacterial strains and plasmids used in gene deletion experiments

E. coli DH5α

F⁻, Ф80d lacZ∆M15, ∆(lacZYA-argF)U169, endA1, recA1, hsdR17(rk⁻, mk⁺) deoR, thi-1, supE44, λ⁻, gyrA96(Nal^r), relA1

Invitrogen

E. coli SM10(λ pir)

thi thr leu tonA lacY supE recA::RP4-2-TC::Mu Km ^r λpir

[53]

B. cenocepacia AU1054

Clinical isolate

BCCM-LMBP

AU1054∆copR

AU1054 derivative with copR deletion

This study

AU1054∆lipA

AU1054 derivative with lipA deletion

This study

PCRII-TOPO

Cloning vector; ori lacZ Km⁺

Invitrogen

pRK2013

ori _colE1, RK2 derivative, Kan^R, mob ⁺, tra ⁺

ATCC

pGPI-SceI

ori _R6K,Tp^R, mob ⁺, carries I-SceI cut site

[37]

pGPI∆copR

pGPI-SceI carrying 5’- and 3’-flanking regions of copR for mutagenesis of copR

This study

pGPI∆lipA

pGPI-SceI carrying 5’- and 3’-flanking regions of lipA for mutagenesis of lipA

This study

pDAI-SceI

ori _pBBR1,Tet^R, mob ⁺, P _dhfr , FLAG epitope, carries I-SceI cut site

[37]

pDA-copR

pDAI-SceI with ORF of copR replacement of SceI gene

This study

pDA-lipA

pDAI-SceI with ORF of lipA replacement of SceI gene

This study