Skip to main content

Table 3 Zeocin-induced mutagenesis in Δpnp strains

From: Polynucleotide phosphorylase is implicated in homologous recombination and DNA repair in Escherichia coli

Strain

Chrom. alleles

Plasmid allele

Fraction surviving (±SD)

Spontaneous mutants (×109)

Zeocin-induced mutants (×109)

Fold induction

Ratio to pnp +

C-1a/pGZ119HE

wild

none

0.40 (±0.014)

273.7 (±44.6)

1116.3 (±125)

4.08

0.95

C-5691/pAZ101

Δpnp

pnp +

0.37 (±0.028)

77.4 (±19.6)

331.4 (±82.8)

4.28

1.00

C-5691/pGZ119HE

Δpnp

none

0.38 (±0.015)

278.2 (±70.8)

530.7 (±98.0)

1.91

0.45

C-5691/pAZ1112

Δpnp

pnpS438A

0.40 (±0.026)

123.9 (±55.2)

222.4 (±85.2)

1.80

0.42

C-5691/pAZ1113

Δpnp

pnp-ΔKH

0.31 (±0.073)

15.8 (±11.7)

108.0 (±75.9)

6.81

1.59

C-5691/pAZ1114

Δpnp

pnp-ΔS1

0.39 (±0.035)

16.7 (±7.8)

119.0 (±55.4)

7.14

1.67

C-5691/pAZ133

Δpnp

pnp-ΔKHS1

0.33 (±0.035)

49.6 (±14.2)

208.4 (77.3)

4.20

0.98

C-5691/pAZ1115

Δpnp

rnb +

0.41 (±0.023)

97.4 (±15.9)

189.3 (±16.2)

1.94

0.45

C-5981/pAZ1115

Δrnb

rnb +

0.39 (±0.029)

48.6 (±23.8)

204.0 (±85.1)

4.20

0.98

C-5981/pGZ119HE

Δrnb

none

0.36 (±0.027)

228.7 (±50.8)

992.5 (±222.8)

4.34

1.01

C-5981/pAZ101

Δrnb

pnp +

0.38 (±0.019)

153.9 (±38.1)

638.4 (±159.4)

4.15

0.97