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Table 3 Zeocin-induced mutagenesis in Δpnp strains

From: Polynucleotide phosphorylase is implicated in homologous recombination and DNA repair in Escherichia coli

Strain Chrom. alleles Plasmid allele Fraction surviving (±SD) Spontaneous mutants (×109) Zeocin-induced mutants (×109) Fold induction Ratio to pnp +
C-1a/pGZ119HE wild none 0.40 (±0.014) 273.7 (±44.6) 1116.3 (±125) 4.08 0.95
C-5691/pAZ101 Δpnp pnp + 0.37 (±0.028) 77.4 (±19.6) 331.4 (±82.8) 4.28 1.00
C-5691/pGZ119HE Δpnp none 0.38 (±0.015) 278.2 (±70.8) 530.7 (±98.0) 1.91 0.45
C-5691/pAZ1112 Δpnp pnpS438A 0.40 (±0.026) 123.9 (±55.2) 222.4 (±85.2) 1.80 0.42
C-5691/pAZ1113 Δpnp pnp-ΔKH 0.31 (±0.073) 15.8 (±11.7) 108.0 (±75.9) 6.81 1.59
C-5691/pAZ1114 Δpnp pnp-ΔS1 0.39 (±0.035) 16.7 (±7.8) 119.0 (±55.4) 7.14 1.67
C-5691/pAZ133 Δpnp pnp-ΔKHS1 0.33 (±0.035) 49.6 (±14.2) 208.4 (77.3) 4.20 0.98
C-5691/pAZ1115 Δpnp rnb + 0.41 (±0.023) 97.4 (±15.9) 189.3 (±16.2) 1.94 0.45
C-5981/pAZ1115 Δrnb rnb + 0.39 (±0.029) 48.6 (±23.8) 204.0 (±85.1) 4.20 0.98
C-5981/pGZ119HE Δrnb none 0.36 (±0.027) 228.7 (±50.8) 992.5 (±222.8) 4.34 1.01
C-5981/pAZ101 Δrnb pnp + 0.38 (±0.019) 153.9 (±38.1) 638.4 (±159.4) 4.15 0.97