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Table 1 Physico-chemical characteristics and microbial populations of barley, oat, triticale and intercropped forages, 90day silages and aerobically exposed silages (n = 3)

From: Bacterial and fungal core microbiomes associated with small grain silages during ensiling and aerobic spoilage

Item

Forage

SEM

P-value*

Silage

SEM

P-value *

Aerobically exposed Silage

SEM

P-value*

Barley

Oat

Triticale

Intercropped

Barley

Oat

Triticale

Intercropped

Barley

Oat

Triticale

Intercropped

pH

7.57b

6.28d

6.52c

6.57c

0.143

<0.001

4.50b

3.98c

3.93c

3.94c

0.070

<0.001

4.39d

8.71b

6.29c

8.43b

0.548

<0.001

DM

48.08b

30.21e

41.65c

38.48d

23.390

<0.001

47.02b

29.87e

39.06c

37.85d

22.060

<0.001

57.88b

35.63d

53.80b

46.95c

2.671

<0.001

DM loss (%)

NM

  

3.10c

1.65c

7.07b

2.11c

0.879

0.009

NM

  

Nutrient composition, % DM basis

 OM

93.61b

92.99c

93.92b

93.11c

0.114

0.002

93.38b

92.58d

92.98c

92.12e

0.144

<0.001

NM

  

 CP

14.20b

11.71c

11.60c

11.91c

0.059

0.003

13.41b

11.48c

13.63b

12.43bc

0.049

0.018

NM

  

 NDF

53.10d

58.73b

51.28d

55.59c

0.878

<0.001

49.34c

57.12b

56.06b

58.00b

1.121

0.003

NM

  

 ADF

27.04d

36.89b

30.51c

30.89c

1.116

0.001

24.89d

34.89b

33.30c

33.14c

1.189

<0.001

NM

  

 ADIN

 (% total N)

NM

  

4.43 bc

5.19b

3.74c

4.78b

0.188

0.013

NM

  

 Starch

21.54b

9.14d

7.41e

10.76c

1.709

<0.001

19.20b

7.04cd

5.54d

8.30c

1.651

<0.001

NM

  

 WSC

 (mg/g DM)

4.66e

31.51d

74.60b

46.33c

7.305

<0.001

0.99c

2.52c

29.20b

12.01bc

7.821

0.031

NM

  

Fermentation products, mg/g DM

 Lactic acid

  

NM

   

44.64e

77.87b

64.56c

52.12d

3.913

<0.001

43.69b

6.60d

28.27c

5.86d

4.935

<0.001

 Acetic acid

  

NM

   

28.92b

17.06c

16.85c

10.32d

1.895

<0.001

26.63b

1.19c

1.33c

0.87c

3.331

<0.001

 Propionic acid

  

NM

   

0.09

ND

ND

ND

NA

NA

0.11

ND

ND

ND

0.015

NA

 Butyric acid

  

NM

   

ND

0.01

ND

0.03

0.031

0.156

ND

0.03b

0.02b

0.02b

0.004

0.021

 La:Ac ratio

  

NM

   

1.54d

4.56b

3.83c

5.05b

0.404

<0.001

1.64c

5.38c

21.42b

6.69c

2.487

0.002

 Total VFAs

  

NM

   

29.09b

17.21c

17.08c

10.42d

1.892

<0.001

26.82b

1.35cd

1.93c

1.07d

3.314

<0.001

 NH3-N

0.13c

0.10d

0.19b

0.11cd

0.011

0.001

2.34b

2.27d

2.13c

1.43d

0.109

<0.001

1.27b

0.22d

1.13b

0.61c

0.134

0.001

Microbiology, log10 CFU/g DM

 Total bacteria

7.71bc

7.47c

7.97b

7.85b

0.085

0.0288

8.92b

8.40c

7.22d

8.05c

0.198

0.0011

7.75d

9.50b

8.78c

9.56b

0.223

<0.001

 LAB

6.23

5.82

6.68

6.98

0.193

0.1267

8.98b

8.52c

7.83d

8.21cd

0.143

0.0017

7.78c

9.37b

9.28b

9.09b

0.216

0.003

 Molds

6.22

6.06

5.99

6.12

0.051

0.4966

ND

ND

ND

ND

NA

NA

ND

8.00b

ND

7.18c

7.180

<0.001

 Yeasts

7.1

7.04

7.09

7.03

0.021

0.4055

<1.00c

5.84b

6.34b

6.75b

0.481

0.0002

4.28c

8.82b

9.19b

8.63b

0.631

0.001

Aerobic Stabilitya

NM

  

NM

  

>336.00d

51.14c

142.91b

39.10c

17.379

0.001

  1. * Within a row, means with a different superscript (b,c,d,e) differ (P < 0.05)
  2. a Hours of aerobic exposure before a 2 °C rise in temperature was recorded in the silage mass after exposure to air
  3. Abbreviations: CP crude protein, NDF neutral detergent fiber, ADF acid detergent fiber, ADIN acid detergent insoluble nitrogen, WSC water soluble carbohydrates, NM not measured, ND not determined, NA not available