Genomic and transcriptomic comparison between Staphylococcus aureus strains associated with high and low within herd prevalence of intra-mammary infection

Table 1 Characteristics of the bacterial strains used in this study

	Herd	IMI prevalence	MLST	RS-PCR	mecA	Virulence profile*
GTS/ST398
Strain 1	A	4%	ST398	GTS	+	lukE, cna, fmtb, scn, chp, lukM
Strain 2	B	2%	ST398	GTS	-	clfA, lukE, cna, fmtb, scn, chp, lukM
Strain 3	C	2%	ST398	GTS	+	clfA, lukE, cna, fmtb, lukM
GTB/ST8
Strain 1	D	49%	ST8	GTB	-	clfA, lukE, cna, sea, sed, sej, fmtb, scn, chp
Strain 2	E	54%	ST8	GTB	-	clfA, lukE, cna, sea, sed, sej, fmtb, scn, chp
Strain 3	F	62%	ST8	GTB	-	clfA, lukE, cna, sed, sej, seg, sei, fmtb, chp

Herd Isolation (six different herds named A-F), IMI prevalence, sequence type characterization by MLST, RS-PCR analysis (genotype S, GTS; genotype B, GTB), mecA detection and virulence genes analysis (*lukE, leucotoxin E gene; cna, collagen adhesin-encoding gene; fmtb, gene encoding for cell wall-associated protein; scn, staphylococcal complement inhibitor gene; chp, chemotaxis inhibitory protein gene; lukM, leukotoxin M gene; clfA, clumping factor A gene; sea, enterotoxin A; sed, enterotoxin D; sej, enterotoxin J; seg, enterotoxin G, sei, enterotoxin I)

ISSN: 1471-2180