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Table 2 Abundance (%) of bacterial mock community at the level of phylum

From: Optimisation of methods for bacterial skin microbiome investigation: primer selection and comparison of the 454 versus MiSeq platform

Taxonomic level Phylum Expected abundance (%) MiSeq platform Roche 454 GS Junior
V1-V3 primer pair V3-V4 primer pair V1-V3 primer pair V3-V4 primer pair
   MBx MHx MBx MHx MBx MHx MBx MHx
Firmicutes 64 94.03 94.02 68.12 69.81 61.44 53.42 75.43 77.70
Proteobacteria 27 5.60 5.79 25.20 23.65 28.46 33.63 18.43 15.12
Actinobacteria 9 0.26 0.18 6.63 6.52 7.18 11.15 5.03 6.39
  1. MBx In-house mock bacterial community with only bacterial DNA, MHx In-house mock bacterial community with human genomic DNA, V1-V3 primer pair primer pair targeting the V1-V3 hypervariable region of the 16S rRNA gene, V3-V4 primer pair primer pair targeting the V3-V4 hypervariable region of the 16S rRNA gene, Roche 454 GS Junior platform pyrosequencing next generation sequencing platform, MiSeq platform sequencing by synthesis next generation sequencing platform