Fig. 4

Bp infection specifically reduces TRAF3 by miR3473 which activates NF-κB-TNF-α pathway. a Protein levels of TRAF3, Phospho-NF-κB p65 and NF-κB p65 in Bp-infected or uninfected macrophages transfected with miR-3473 mimic, inhibitor or controls, as assessed by western blot with Actin as an internal control. b Protein levels in Bt-infected or uninfected RAW264.7 cell were tested as Fig. 4a proceed. c qRT-PCR analysis of TRAF3 mRNA in TRAF3-overexpressed and negative control macrophages, with actin as an internal control. d TNF-α mRNA was evaluated in TRAF3-overexpressed or untransfected macrophages at 12 h after Bp infection. *P value < 0.05, **P value < 0.01