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Table 1 Vaginal human microbiome analysis

From: Evaluation of PacBio sequencing for full-length bacterial 16S rRNA gene classification

  HQR OTUs 97% OTUs 98% OTUs 99% OTUs unique
PacBio V1V9 Minscore = 90 minsim = 90 20959 60 (48) 63 (49) 90 (62) 209 (148)
PacBio V1V9 Minscore = 80 minsim = 80 23701 181 (83) 453 (136) 1109 (158) 1316 (261)
PacBio in silico V1V2 Minscore = 90 Minsim = 90 26765 82 (60) 131 (65) 426 (113) 486 (131)
PacBio in silico V1V2 Minscore = 80 Minsim = 80 27596 160 (81) 328 (103) 729 (137) 802 (154)
MiSeq V1V2 508016 419 (213) 536 (269) 691 (316) 829 (382)
MiSeq V1V2 Subsampled to 27596 27596 130 (79) 152 (89) 168 (94) 219 (129)
  1. HQR high quality reads, V1V9 full-length bacterial 16S gene, V1V2 bacterial 16S V1V2 gene region, minscore = minimum alignment score during post alignment screening, minsim = minimum similarity score during post alignment screening, OTU operational taxonomic units, value in round brackets number of singleton-free OTUs (OTUs with 1 sequence across all groups were removed)