Table 1 Vaginal human microbiome analysis
From: Evaluation of PacBio sequencing for full-length bacterial 16S rRNA gene classification
HQR | OTUs 97% | OTUs 98% | OTUs 99% | OTUs unique | |
|---|---|---|---|---|---|
PacBio V1V9 Minscore = 90 minsim = 90 | 20959 | 60 (48) | 63 (49) | 90 (62) | 209 (148) |
PacBio V1V9 Minscore = 80 minsim = 80 | 23701 | 181 (83) | 453 (136) | 1109 (158) | 1316 (261) |
PacBio in silico V1V2 Minscore = 90 Minsim = 90 | 26765 | 82 (60) | 131 (65) | 426 (113) | 486 (131) |
PacBio in silico V1V2 Minscore = 80 Minsim = 80 | 27596 | 160 (81) | 328 (103) | 729 (137) | 802 (154) |
MiSeq V1V2 | 508016 | 419 (213) | 536 (269) | 691 (316) | 829 (382) |
MiSeq V1V2 Subsampled to 27596 | 27596 | 130 (79) | 152 (89) | 168 (94) | 219 (129) |