Table 1 Primer sequences
From: Pyruvate oxidase of Streptococcus pneumoniae contributes to pneumolysin release
Primer name | Sequence (5′-3′) |
|---|---|
LytA-KO1 | GCGGGTACCCAGTCCAGCTTTGGTTTCCT |
LytASup | TAAAAATATCTCTTGCCAGTCCTTGCCTATATGGTTGCACG |
LytASdn | GGTAATCAGATTTTAGAAAACAATAAACCCTCACAGTAGAGCCAGAT |
LytA-KO4 | CGCGGATCCTCACAGTAGAGCCAGATGGC |
PlyAF | CTCAATCCAGCTACCTGTCGC |
PlyAR | GTTTGCTTCTAAGTCTTATTTCCCTTCTACCTCCTAATAAG |
PlyBF | GAGTCGCTTTTGTAAATTTGGGAGAGGAGAATGCTTGCG |
PlyBR | GCTTGTTTAGCACGGTCG |
ALR1 | GCG GGTACCGCGTGCTATTGCAGATCAAA |
ALR2 | GCGTCTAGACATCGTTAATCGGAGATGGA |
ALR3 | CGCGGATCCATCTACGCCCCATGTTTTCAATACG |
ALR4 | CGCGGATCCACCATTCCGTCTCTTCTTGG |
ALR5 | CGCGGATCCGGAAATAAGACTTAGAAGCAAAC |
ALR6 | CGCGGATCCCCAAATTTACAAAAGCGACTC |
SpxB-F | CGCGCCCGGGTGACAACACTTTCAAAACTG |
SpxB-R | CGCGGAATTCTTATTTAATTGCGCGTGATTGC |
ERM-F | GGAAATAAGACTTAGAAGCAAAC |
ERM-R | CCAAATTTACAAAAGCGACTC |
Spec-F | CGTGACTGGCAAGAGATATTTTTA |
Spec-R | GGGTTTATTGTTTTCTAAAATCTGATTACC |
PLY-F | CAGAGCGTCCTTTGGTCTATATT |
PLY-R | CAGCCTCTACTTCATCACTCTTAC |