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Table 1 Summary of phenotypes for isolates that were recovered from biofilm of MC1000

From: Spontaneous mutations in the flhD operon generate motility heterogeneity in Escherichia coli biofilm

Incubation # of col. Motilitya Colony designationsb pXL27 compl.c PCR1/PCR2d PCR3e
Partially Motile Non-Motile
Category I Category II Category III
7d 368 4 NM (1 %) JS28-31 4 col. PM   4 col. with parental PCR fragments    ND
9 PM (2.4 %) JS19-JS27 ND 9 col. with parental PCR fragments     ND
14 d 1217 58 NM (4.8 %) JS18, JS42-79, JS82-JS100 54 col. FM, 4 col. PM   21 col. with parental PCR fragments 3 col. lacking a PCR1 product, 3 col. lacking a PCR2 product 31 col. with extended PCR fragments in both reactions 31 col. tested: 6 col. JS44 orient, 22 col. JS90 orient, 3 col. no PCR3 fragments
14 PM (1.1 %) S17, JS32-JS41, JS80, JS81, JS101 ND 14 col. with parental PCR fragments     ND
  1. aMotility was determined on motility plates: NM, the isolate was completely non-motile; PM, the isolate exhibited partial motility relative to its parent strain
  2. bAll 85 MC1000 biofilm isolates that exhibited reduced motility relative to MC1000 were given JS designations
  3. c Non-motile isolates from MC1000 biofilm were tested for complementation with pXL27: FM, at least 7 of 8 transformants exhibited the full motility of the parent; PM, fewer than 7 of 8 transformants were fully motile or all colonies were partially motile. ND, not determined
  4. dReduced motility isolates from MC1000 biofilm were subjected to PCR1 and PCR2 to test for insertions/deletions within the flhD operon
  5. eThe 31 isolates from category III were subjected to PCR3 to identify IS1 insertions