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Table 1 Kinetic constants of YcsE

From: Bacillus subtilis 5′-nucleotidases with various functions and substrate specificities

Substrate K M (mM)a V max (μmol min−1 mg protein−1)a k cat (s−1)a
IMP 3.2 ± 0.036 0.13 ± 0.060 3.7 ± 1.4 × 102
AMP 8.6 ± 0.80 0.50 ± 0.029 2.3 ± 0.13 × 103
GMP 10.3 ± 0.12 0.45 ± 0.12 2.0 ± 0.55 × 103
CMP 4.7 ± 0.23 0.53 ± 0.18 3.4 ± 0.011 × 103
UMP 8.5 ± 0.69 0.20 ± 0.072 9.6 ± 3.5 × 102
G6P 22.2 ± 0.96 2.3 ± 0.48 1.06 ± 0.22 × 104
  1. aAll presented data are the mean values of three independent experiments ± SD