Table 1 Bacterial strains and plasmids used in this study
Strain/plasmid | Genotype or description | Source |
|---|---|---|
Strains | ||
S. pneumoniae | ||
Sp1 (Rx1) | unencapsulated wild-type, str1; hexA | [54] |
Sp10 | Cm, stkP::cm | [17] |
Sp26 | Cm, divIVA::cm | [55] |
Sp57 | locZ::lox72 | [25] |
Sp100 | Kan, phpP::kan rpsL | This work |
Sp113 | ΔphpP | This work |
Sp120 | Tet, ΔphpP bga::PZn-phpP | This work |
Sp140 | Tet, ΔphpP bga::PZn-gfp-phpP | This work |
Sp161 | Kan, ΔphpP divIVA::kan rpsL | This work |
Sp169 | ΔphpP ΔdivIVA | This work |
Sp174 | Erm, ΔdivIVA pMU-P96-divIVA-flag | This work |
Sp188 | Erm, ΔlocZ pMU-P96-flag-locZ | This work |
Sp220 | Kan, phpP::kan rpsL (reverted from Sp113) | This work |
Sp222 | wild-type (reverted from Sp113) | This work |
Sp292 | Tet, ΔphpP bga::PZn -gfp-phpP-D192A | This work |
Sp293 | Tet, ΔphpP bga::PZn -gfp-phpP-D231A | This work |
Sp295 | Δjag | This work |
Sp302 | Tet, Δjag bga::PZn -jag-flag-T89A | This work |
Sp304 | Tet, Δjag bga::PZn -jag-flag | This work |
E. coli | ||
DH5α | F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rk-, mk+) phoA supE44 λ- thi-1 gyrA96 relA1 | Invitrogen |
BL21 | F- ompT gal [dcm][lon] hsdSB (rB- mB-) (DE3) | Novagen |
Plasmids | ||
pJWV25 | Amp, tet, bgaA, PZn-gfp+ | [35] |
pZn-PhpP | Amp, tet, bgaA, PZn-phpP | This work |
pZn-flag-locZ | Amp, tet, bgaA, PZn -flag-locZ | This work |
pJWV25-phpP | Amp, tet, bgaA, PZn-gfp-phpP | [20] |
pZn-gfp-phpP-D192A | Amp, tet, bgaA, PZn -gfp-phpP-D192A | This work |
pZn-gfp-phpP-D231A | Amp, tet, bgaA, PZn -gfp-phpP-D231A | This work |
pZn-jag-flag-T89A | Amp, tet, bgaA, PZn -jag-flag-T89A | This work |
pZn-jag-flag | Amp, tet, bgaA, PZn -jag-flag | This work |
pEXphpP-D231A | Kan, phpP-D231A | [17] |
pMU1328 | Erm, empty vector | [51] |
pMU-P96-divIVA-flag | Erm, P96-divIVA-flag | This work |
pMU-P96-flag-locZ | Erm, P96-flag-locZ | This work |
Janus cassette | Kan, kan-rpsL + | [52] |