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Table 1 Bacterial strains and plasmids used in this study

From: Characterization of pneumococcal Ser/Thr protein phosphatase phpP mutant and identification of a novel PhpP substrate, putative RNA binding protein Jag

Strain/plasmid Genotype or description Source
Strains
  S. pneumoniae
  Sp1 (Rx1) unencapsulated wild-type, str1; hexA [54]
  Sp10 Cm, stkP::cm [17]
  Sp26 Cm, divIVA::cm [55]
  Sp57 locZ::lox72 [25]
  Sp100 Kan, phpP::kan rpsL This work
  Sp113 ΔphpP This work
  Sp120 Tet, ΔphpP bga::PZn-phpP This work
  Sp140 Tet, ΔphpP bga::PZn-gfp-phpP This work
  Sp161 Kan, ΔphpP divIVA::kan rpsL This work
  Sp169 ΔphpP ΔdivIVA This work
  Sp174 Erm, ΔdivIVA pMU-P96-divIVA-flag This work
  Sp188 Erm, ΔlocZ pMU-P96-flag-locZ This work
  Sp220 Kan, phpP::kan rpsL (reverted from Sp113) This work
  Sp222 wild-type (reverted from Sp113) This work
  Sp292 Tet, ΔphpP bga::PZn -gfp-phpP-D192A This work
  Sp293 Tet, ΔphpP bga::PZn -gfp-phpP-D231A This work
  Sp295 Δjag This work
  Sp302 Tet, Δjag bga::PZn -jag-flag-T89A This work
  Sp304 Tet, Δjag bga::PZn -jag-flag This work
  E. coli
  DH5α F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rk-, mk+) phoA supE44 λ- thi-1 gyrA96 relA1 Invitrogen
  BL21 F- ompT gal [dcm][lon] hsdSB (rB- mB-) (DE3) Novagen
Plasmids
  pJWV25 Amp, tet, bgaA, PZn-gfp+ [35]
  pZn-PhpP Amp, tet, bgaA, PZn-phpP This work
  pZn-flag-locZ Amp, tet, bgaA, PZn -flag-locZ This work
  pJWV25-phpP Amp, tet, bgaA, PZn-gfp-phpP [20]
  pZn-gfp-phpP-D192A Amp, tet, bgaA, PZn -gfp-phpP-D192A This work
  pZn-gfp-phpP-D231A Amp, tet, bgaA, PZn -gfp-phpP-D231A This work
  pZn-jag-flag-T89A Amp, tet, bgaA, PZn -jag-flag-T89A This work
  pZn-jag-flag Amp, tet, bgaA, PZn -jag-flag This work
  pEXphpP-D231A Kan, phpP-D231A [17]
  pMU1328 Erm, empty vector [51]
  pMU-P96-divIVA-flag Erm, P96-divIVA-flag This work
  pMU-P96-flag-locZ Erm, P96-flag-locZ This work
  Janus cassette Kan, kan-rpsL + [52]
  1. Amp ampicillin resistance marker, cm chloramphenicol resistance marker, kan kanamycin resistance marker, tet tetracycline resistance marker, erm erythromycin resistance marker
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BMC Microbiology

ISSN: 1471-2180

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