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Table 2 Results of 454 pyrosequencing from each Red Snapper individual and sample type

From: Microbiota of wild-caught Red Snapper Lutjanus campechanus

Fish ID

Sample type

Original # sequences

Original # OTUs

Final # sequences

Final # OTUs

Expected # OTUs

Good's coverage

Shannon evennness index

01B

Blood

2263

48

950

43

56

0.991

0.724

04B

Blood

3019

83

950

64

103

0.98

0.735

08B

Blood

950

49

950

49

99

0.991

0.712

10B

Blood

2495

52

950

46

62

0.988

0.665

01G

Gill

3055

74

950

59

103

0.984

0.599

02G

Gill

2581

80

950

68

104

0.981

0.602

03G

Gill

9276

91

950

51

102

0.984

0.533

04G

Gill

3045

81

950

61

101

0.982

0.455

05G

Gill

11888

153

950

90

245

0.963

0.689

06G

Gill

4000

134

950

90

170

0.973

0.762

07G

Gill

4170

109

950

75

247

0.983

0.816

08G

Gill

3140

77

950

64

107

0.986

0.706

09G

Gill

4355

76

950

54

121

0.986

0.649

05 F

Feces

3859

109

950

74

147

0.973

0.545

06 F

Feces

3669

141

950

99

195

0.967

0.704

07 F

Feces

4156

92

950

66

141

0.987

0.8

08 F

Feces

4626

116

950

72

354

0.966

0.512

09 F

Feces

2595

66

950

53

126

0.986

0.727

10 F

Feces

3154

107

950

80

251

0.968

0.591

Average

Blood

2182

58

950

51

80

0.988

0.709

 

Gill

5057

97

950

68

144

0.98

0.646

 

Feces

3677

105

950

74

202

0.975

0.647

  1. Averages for each sample type are included. Original sequences and # OTUs are from non-standardized data whereas final sequences and # OTUs are after standardizing to 950 sequences per sample (sample 08B)