Fig. 3

L. salivarius strains suppress H. pylori-activated NF-κB in AGS gastric epithelial cells. H. pylori-stimulated AGS cells were incubated with medium control or LCM of LS-B37 and LS-B60 for 0.25, 0.5, 1, 2, and 3 h. Concentration of activated NF-κB were measured by Western blot analysis using antibodies to p-NF-κB p65 and NF-κB p65. Relative protein concentration was quantified by densitometry and activated transcription factor (p-NF-κB p65 [Ser 536]) was normalized to non-activated counterpart (NF-κB p65). The results were from three independent experiments and expressed as the mean ± SEM, **p < 0.01 as compared to medium control