Fig. 2From: TLR9 mediates S. aureus killing inside osteoblasts via induction of oxidative stressCpG-ODNs induce the expression of TLR9 in SAOS-2 cells. SAOS-2 cells were treated for 4Â h with 250 nM of ODN2216 (1), ODN2006 (2), ODN2243 (3) or left untreated (4). cDNA was synthetized from isolated SAOS-2 RNA and underwent real-time quantitative PCR using human TLR9 or ACTB primers. PCR products were analyzed on 1Â % agarose gelBack to article page