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Table 4 Differential gene expression in lipid accumulation related pathways

From: De novo transcriptomic analysis of Chlorella sorokiniana reveals differential genes expression in photosynthetic carbon fixation and lipid production

Product Gene name EC number Trinity ID Log2FCa (48 h) Log2FC (84 h)
Fatty acid biosynthyesis pathway
acetyl-CoA carboxylase ACC EC:6.4.1.2 comp6756_c0_seq1 −1.71 −0.63
biotin carboxylase BC EC:6.3.4.14 comp12323_c26_seq1 0.82 2.55
malonyl-CoA ACP transacylase MAT EC:2.3.1.39 comp11367_c0_seq2 −1.27 −0.74
KAS Beta-ketoacyl-ACP synthase KASII EC:2.3.1.179 comp11672_c1_seq3 0.48 0.72
KAS Beta-ketoacyl-ACP synthase KASIII EC:2.3.1.180 comp10141_c0_seq2 0.27 0.35
Beta-ketoacyl-ACP reductase KAR EC:1.1.1.100 comp10720_c0_seq1 1.23 0.74
beta-hydroxyacyl-ACP dehydrase HAD EC:4.2.1.59 comp12130_c0_seq1 0.31 1.43
Enoyl-ACP reductase EAR EC:1.3.1.10 comp5575_c1_seq1 −2.23 0.10
acyl-ACP desaturase AAD EC:1.14.19.2 comp12618_c0_seq1 0.27 0.45
Oleoyl-ACP thioesterase OAT EC:3.1.2.14 comp10287_c1_seq1 0.54 0.53
Triacylglycerol biosynthesis pathway
glycerol kinase GK EC:2.7.1.30 comp17390_c0_seq1 1.21 1.12
glycerol-3-phosphate O-acyltransferase GPAT EC:2.3.1.15 comp8649_c0_seq4 0.14 2.41
1-acyl-sn-glycerol-3-phosphate acyltransferase AGPAT EC:2.3.1.51 comp8576_c1_seq1 1.05 0.99
phosphatidate phosphatase PP EC:3.1.3.4 comp10073_c0_seq1 −0.75 −3.36
diacylglycerol O-acyltransferase DGAT EC:2.3.1.20 comp8627_c0_seq2 0.08 0.15
Fatty acid catabolism pathway
acyl-CoA synthetase ACSL EC:6.2.1.3 comp11861_c1_seq8 −0.57 −0.29
acyl-CoA oxidase AOx EC:1.3.3.6 comp8247_c0_seq2 2.17 1.24
acyl-CoA dehydrogenase ACDH EC:1.3.8.7 comp12261_c15_seq6 0.62 1.22
enoyl-CoA hydratase ECH EC:4.2.1.17 comp8664_c0_seq1 0.46 0.27
3-hydroxyacyl-CoA dehydrogenase HADH EC:1.1.1.35, 1.1.1.211 comp13763_c0_seq1 1.64 1.97
acetyl-CoA C-acyltransferase ACAT EC:2.3.1.16, 2.3.1.9 comp12241_c5_seq15 0.89 0.02
Starch biosynthesis and catabolism, and ethanol fermentation pathway
phosphoglucomutase PGM EC:5.4.2.2 comp10999_c2_seq3 −1.11 −0.12
ADP-glucose pyrophosphorylase AGPase EC:2.7.7.27 comp11635_c1_seq2 0.30 1.67
starch synthase SS EC:2.4.1.21 comp13246_c0_seq1 0.04 0.30
1,4-α-glucan branching enzyme BE EC:2.4.1.18 comp7752_c1_seq1 −1.47 −1.66
hexokinase HXK EC:2.7.1.1 comp5345_c0_seq1 −0.20 1.49
β-amylase β-AMY EC:3.2.1.2 comp13107_c0_seq1 1.49 1.36
α-amylase α-AMY EC:3.2.1.1 comp11995_c1_seq7 2.22 1.10
oligo-1,6-glucosidase O1,6G EC:3.2.1.10 comp11995_c1_seq11 2.20 1.49
starch phosphorylase Spase EC:2.4.1.1 comp12085_c7_seq13 0.79 1.47
pyruvate decarboxylase PDC EC:4.1.1.1 comp6820_c0_seq2 −0.30 0.93
alcohol dehydrogenase ADH EC:1.1.1.1 comp11611_c1_seq3 0.71 1.13
Pyruvate dehydrogenase complex PDHC EC:1.2.4.1, 2.3.1.12, 1.8.1.4 comp12893_c0_seq1 1.48 1.90
  1. a \( \mathrm{Log}2\mathrm{F}\mathrm{C}= Log2\left(\frac{FPKMnitrogen- limited}{FPKMnitrogen- replete}\right) \)