Table 4 Hit rate analysis for the GBS RPA and real-time PCR assays showing number of replicates of each target input concentration detected
Target input genome copies | No replicates tested | No replicates detected in RPA assay | No replicates detected in PCR assay |
|---|---|---|---|
100 | 24 | 24 | 24 |
50 | 24 | 24 | 24 |
25 | 24 | 24 | 24 |
12.5 | 24 | 24 | 24 |
6.25 | 24 | 20 | 24 |
3.1 | 24 | 15 | 14 |
1.5 | 24 | 14 | 18 |
0.8 | 24 | 10 | 9 |