Fig. 5
Adhesion of labeled B. pertussis Tohama I on polarized Calu-3 cells at air-liquid interface. a Polarized Calu-3 cells were infected with labeled B. pertussis Tohama I at OD600 0.4 for 1 h. After extensive washes, infected cells were fixed and tight junctions (ZO-1, red) and nuclei (DAPI, blue) were stained for confocal microscopy analysis. Images are 3D view of Z-stack acquisition. b Polarized Calu-3 cells were infected for 1 h with serially diluted labeled bacteria starting from an OD600 of 0.4. After washes, cell-associated bacteria were quantified by fluorescence reading at Ex/Em 485/535 nm. Results represent mean ± SD from a representative experiment performed in triplicates