|
acrAB, tolC, ybaB, ksgA, yebC, smpB (0.73) a
|
Affect kanamycin resistance [35–37]
|
|
dnaQ, holD, recD, xseA, ruvA (0.58), ruvB (0.60), ruvC (0.61), recB (0.58), recC (0.65)
|
Likely to affect the transposition process; dnaQ, holD, ruvA, and ruvB inferred as essential using Tn-seq in Salmonella [14]
|
|
priB
|
Deficient in plasmid maintenance [63, 64]
|
|
fhuACD, tonB
|
Involved in iron acquisition which is critical for growth in the iron limited media used in this study [33]
|
|
rpsT
|
S20 ribosomal subunit; new data indicates mutants have poor growth [42] (in conflict with Keio data)
|
|
miaA
|
tRNA dimethyl transferase; previous data indicates E. coli mutants have poor growth [43] (in conflict with Keio data)
|
|
ompA
|
Outer membrane porin; clean knockouts appear viable [65]; mutant forms are frequently lethal [44]
|
|
pitA
|
Metal phosphate transporter with ten transmembrane segments; transposon disruption of substrate transporters is three-fold more likely to be inferred as essential compared to clean deletion (see main text; Fig. 5)
|
|
potB
|
Type I ABC transporter (Putrescine / spermidine transporter); see above
|
|
sapB
|
Type I ABC transporter (unknown substrate); see above
|
|
ptsH, yeaC, ydhR
|
Short reading frames (306 bp, 272 bp, and 258 bp, respectively)
|
