Fig. 7

Results of co-culturing M. producens JHB with various other laboratory cultures to evaluate the transferability of Mor1. Shown are 16S rRNA and selA PCR reactions using DNA from co-culturing of M. producens JHB with other cyanobacteria, visualized on 1 % agar gels. a The 16S rRNA PCR of all samples. b The selA PCR of all samples. Both gels were loaded with the same order of samples for each respective PCR reaction. Lane 1: Molecular weight marker (Invitrogen 1 kb Plus DNA Ladder). Lane 2: Negative control, sterile water. Lane 3: M. producens JHB. Lane 4: 3 L Oscillatoria. Lane 5: PAP25Jun12-2. Lane 6: Leptolyngbya sp. Lanes 7 and 8: 3 L Oscillatoria from co-culture with JHB, duplicate co-cultures. Lanes 9 and 10: PAP25Jun12-2 from co-culture with JHB, duplicate co-cultures. 1 Lanes 11 and 12: Leptolyngbya sp. from co-culture with JHB, duplicate co-cultures. Lane 13: PAP25Jun12-2 from co-culture with Leptolyngbya sp. Lane 14: PAP25Jun12-2 from co-culture with 3 L Oscillatoria. Lane 15: Leptolyngbya sp. from co-culture with PAP25Jun12-2. Lane 16: Leptolyngbya sp. from co-culture with 3 L Oscillatoria. Lane 17: 3 L Oscillatoria from co-culture with PAP25Jun12-2. Lane18: 3 L Oscillatoria from co-culture with Leptolyngbya sp