Table 3 Ct-values of the three DNA extraction protocols of the MTC-specific target genes Heli and IS 1081 generated with the field tissue samples of red deer
Animal No. | Tissue | Weight in grams | Protocol 1 | Protocol 2 | Protocol 3 | Protocol 1 | Protocol 2 | Protocol 3 | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
Heli | IS 1081 | Heli | IS 1081 | Heli | IS 1081 | Overall result per animal | |||||
1 | Intestinal ln | 5 | no ct | no ct | no ct | no ct | – | – | negative | negative | – |
2 | Intestinal ln | 3 | no ct | no ct | no ct | no ct | no ct | no ct | negative | negative | negative |
3 | Mesenteric ln | 2 | no ct | no ct | no ct | no ct | – | – | negative | negative | – |
4 | Intestinal ln | 8 | no ct | no ct | no ct | no ct | – | – | negative | negative | – |
5 | Intestinal ln | 3 | no ct | no ct | no Ct | no Ct | – | – | negative | negative | – |
6 | Peritoneum | 2 | no ct | no ct | no Ct | no Ct | – | – | negative | negative | – |
7 | Intestinal ln | 4 | no ct | no ct | no Ct | no Ct | 41.90 | no ct | negative | negative | negative |
8 | Mesenteric ln | 3 | 35.63 | 34.79 | ne | ne | – | – | positive | – | – |
9 | Intestinal ln | 3 | no ct | 37.57 | ne | ne | – | – | positive | positive | – |
Lung | 5 | 26.27 | 26.14 | 27.00 | 27.20 | – | – | ||||
10 | Intestinal ln | 2 | no ct | no ct | no Ct | no Ct | – | – | negative | negative | – |
Lung | 3 | no ct | no ct | ne | ne | – | – | ||||
11 | Intestinal ln | 19 | 33.78 | 35.20 | 35.02 | 35.53 | 33.11 | 33.60 | positive | positive | positive |
Retropharyngeal ln | 3 | no ct | 39.54 | 38.09 | 37.96 | 41.77 | 41.11 | ||||
12 | Intestinal ln | 5 | 37.54 | 36.79 | 35.67 | 36.38 | 37.82 | 38.08 | positive | positive | positive |
Retropharyngeal ln | 11 | no ct | no ct | 38.82 | 39.81 | 37.03 | 36.81 | ||||
13 | Lung | 7 | 33.74 | 34.62 | 35.90 | 36.20 | – | – | positive | positive | – |
Pleura | 2 | 37.14 | 37.07 | 36.10 | 37.20 | ||||||
Palatine tonsil | 3 | 35.49 | 36.08 | 33.20 | 33.10 | ||||||
Mass | 8 | 34.03 | 38.53 | 32.80 | 33.10 | ||||||
Diaphragm | 11 | 31.85 | 31.84 | 38.10 | 37.60 | ||||||
14 | Lung | 3 | no ct | no ct | no Ct | no Ct | no ct | no ct | negative | negative | negative |
15 | Retropharyngeal ln | 8 | no ct | no ct | no Ct | no Ct | no ct | 39.92 | negative | negative | negative |
16 | Intestinal ln | 2 | no ct | no ct | ne | ne | no ct | no ct | negative | – | negative |
17 | Retropharyngeal lna | 5 | 17.95 | 25.95 | 26.88 | 27.61 | 25.91 | 26.25 | positive | positive | positive |
18 | Intestinal ln | 2 | no ct | no ct | no Ct | no Ct | no ct | no ct | negative | negative | negative |