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Table 3 Ct-values of the three DNA extraction protocols of the MTC-specific target genes Heli and IS 1081 generated with the field tissue samples of red deer

From: Two alternative DNA extraction methods to improve the detection of Mycobacterium-tuberculosis-complex members in cattle and red deer tissue samples

Animal No. Tissue Weight in grams Protocol 1 Protocol 2 Protocol 3 Protocol 1 Protocol 2 Protocol 3
Heli IS 1081 Heli IS 1081 Heli IS 1081 Overall result per animal
1 Intestinal ln 5 no ct no ct no ct no ct negative negative
2 Intestinal ln 3 no ct no ct no ct no ct no ct no ct negative negative negative
3 Mesenteric ln 2 no ct no ct no ct no ct negative negative
4 Intestinal ln 8 no ct no ct no ct no ct negative negative
5 Intestinal ln 3 no ct no ct no Ct no Ct negative negative
6 Peritoneum 2 no ct no ct no Ct no Ct negative negative
7 Intestinal ln 4 no ct no ct no Ct no Ct 41.90 no ct negative negative negative
8 Mesenteric ln 3 35.63 34.79 ne ne positive
9 Intestinal ln 3 no ct 37.57 ne ne positive positive
Lung 5 26.27 26.14 27.00 27.20
10 Intestinal ln 2 no ct no ct no Ct no Ct negative negative
Lung 3 no ct no ct ne ne
11 Intestinal ln 19 33.78 35.20 35.02 35.53 33.11 33.60 positive positive positive
Retropharyngeal ln 3 no ct 39.54 38.09 37.96 41.77 41.11
12 Intestinal ln 5 37.54 36.79 35.67 36.38 37.82 38.08 positive positive positive
Retropharyngeal ln 11 no ct no ct 38.82 39.81 37.03 36.81
13 Lung 7 33.74 34.62 35.90 36.20 positive positive
Pleura 2 37.14 37.07 36.10 37.20   
Palatine tonsil 3 35.49 36.08 33.20 33.10   
Mass 8 34.03 38.53 32.80 33.10   
Diaphragm 11 31.85 31.84 38.10 37.60   
14 Lung 3 no ct no ct no Ct no Ct no ct no ct negative negative negative
15 Retropharyngeal ln 8 no ct no ct no Ct no Ct no ct 39.92 negative negative negative
16 Intestinal ln 2 no ct no ct ne ne no ct no ct negative negative
17 Retropharyngeal lna 5 17.95 25.95 26.88 27.61 25.91 26.25 positive positive positive
18 Intestinal ln 2 no ct no ct no Ct no Ct no ct no ct negative negative negative
  1. ln lymph node, ne not evaluable
  2. – not done
  3. amicroscopic examination of Ziehl-Neelsen-stained sediments