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Table 1 Oligonucleotide sequences of capture-oligonucleotides, real-time PCR primers and probes

From: Two alternative DNA extraction methods to improve the detection of Mycobacterium-tuberculosis-complex members in cattle and red deer tissue samples

No.   Name Sequence 5′-3′ 5′-label 3′-label
1 oligonucleotides for capturing MTC DNA:
Cap 1 and Cap 2 are specific for Heli; Cap 3 and Cap 4 are specific for IS 1081
Cap 1 TTG ATC AGG TCG ACG ATG TAG Biotin TEG  
Cap 2 TCA CCA CCG ACA AAG CGT C Biotin TEG  
Cap 3 CTC TCG ACG TTC ATC GCC G Biotin TEG  
Cap 4 TGG CGG TAG CCG TTG CGC Biotin TEG  
2 duplex real-time PCR specific for the hypothetical Helicase (Heli) MTC-Heli 4 F TTG ATC AGG TCG ACG ATG TAG   
MTC Heli 4R TCA CCA CCG ACA AAG CGT C   
MTC Heli 4 FAM TCA ACG ACC CCA ACG ACT GGT GC FAM BHQ1
3 duplex real-time PCR specific for the insertion sequence 1081 (IS 1081) MTC IS 1081 5 F CTC TCG ACG TTC ATC GCC G   
MTC IS 1081 5R TGG CGG TAG CCG TTG CGC   
MTC IS 1081 5 FAM ATT GGA CCG CTC ATC GCT GCG TTC FAM BHQ1
4 Duplex real-time PCR specific for ß-actin ACT2 1030 F AGC GCA AGT ACT CCG TGT G   
ACT 1135 R CGG ACT CAT CGT ACT CCT GCT T   
ACT-1081 1105 Y TCG CTG TCC ACC TTC CAG CAG ATGT YakimaYellow BHQ1
  1. Primer and Probes, no. 2, 3 and 4 according to the Official Collection of Methods [19]