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Table 1 Comparison of CFU and acid/HPLC to quantify the amount of DNA in bacteria

From: A method for assessing efficiency of bacterial cell disruption and DNA release

Microorganism

DNA content (μg) based on CFU

DNA content (μg) based on acid/HPLC

B. subtilis

1.32 ± 0.034 (n = 3)a

1.62 ± 0.029 (n = 3)

M. smegmatis

6.10 ± 0.906 (n = 5)b

15.91 ± 0.338 (n = 5)

  1. aAssumes that Bacillus subtilis contains 4.9 μg DNA/109 cells
  2. bAssumes that Mycobacterium smegmatis contains 8.2 μg DNA/109 cells