Skip to main content

Table 1 Comparison of CFU and acid/HPLC to quantify the amount of DNA in bacteria

From: A method for assessing efficiency of bacterial cell disruption and DNA release

Microorganism DNA content (μg) based on CFU DNA content (μg) based on acid/HPLC
B. subtilis 1.32 ± 0.034 (n = 3)a 1.62 ± 0.029 (n = 3)
M. smegmatis 6.10 ± 0.906 (n = 5)b 15.91 ± 0.338 (n = 5)
  1. aAssumes that Bacillus subtilis contains 4.9 μg DNA/109 cells
  2. bAssumes that Mycobacterium smegmatis contains 8.2 μg DNA/109 cells