Diversity of Clostridium perfringens toxin-genotypes from dairy farms

BMC Microbiology

Table 1 Oligonucleotide primer for 16S rRNA gene amplification and sequencing

Primer	Sequence (5′–3′)	Location^c
16S rRNA gene amplification primer^a
16SUNI-L	AGAGTTTGATCATGGCTCAG	8-27
16SUNI-R	GTGTGACGGGCGGTGTGTAC	1410-1391
16S rRNA gene sequencing primer^b
533F	GTGCCAGCAGCCGCGGTAA	514-532
907R	CCGTCAATTCMTTTGAGTTT	925-906

^aKuhnert P, Capaul SE, Nicolet J, Frey J. Phylogenetic positions of Clostridium chauvoei and Clostridium septicum based on 16S rRNA gene sequences. Int J Syst Bacteriol. 1996;46:1174-1176
^bPetti CA. Detection and identification of microorganisms by gene amplification and sequencing. Clin Infect Dis. 2007;44:1108–1114
^cIndicating the position of each primer relative to the 16S rRNA gene sequence of E. coli J01859

ISSN: 1471-2180